Hi!
I see that many people use ERCC spike-ins with prokaryotes, but looking at the manufacturer description I see that they only mention that it...
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ERCC spike-ins for prokaryotes
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Upcoming Webinar – Controlling RNA-Seq Experiments Using Spike-In RNA Variants
DATE: We, October 19th, 2016
TIME: 9:00 AM PDT, 12:00 PM EDT, 6:00 PM CEST
PRESENTED BY: Lukas Paul, PhD
Head of Services,...
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SIRVs (Spike-In RNA Variants): external controls for RNA sequencing
With the invigorated interest in NGS spike-ins we want to focus the posts dealing with Lexogen's SIRVs controls in this thread. While Lexogen will annouce...
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Truseq libray spike-in into Nextera library run
Hi.
In our lab we routinly perform exome sequencing with Nextera kits on Mi/HiSeq platforms.
In my experiment I want to sequence...
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Spike-in sets for small RNA seq?
I'm thinking of making a commercial offering of the SRQC and ERDN spike-in sets for small RNASeq: http://nar.oxfordjournals.org/content/early/2015/04...
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how to extract miRNA spike-in controls from smallRNAseq data
Hi,
I'm processing single end Illumina Hiseq2000 miRNA data. I've got 5 miRNA spike-in controls in my samples. How could I extract those from the...
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ERCC ExFold vs. regular spike-in mix?
Does anyone know the practical difference between the Ambion ERCC RNA Spike-In Mix (Life Tech #4456740) and their ExFold mixes (#4456739)? The regular...
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DESeq analysis with ERCC RNA spike ins
Hi, I want to perform differential expression analyses of multiple RNA-seq samples using DESeq. We have included ERCC spike in controls and wish to use...
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Question about SAMstrt Spike-in RNA
hi SEQers~
I recently read SAMstrt: statistical test for differential expression in single-cell transcriptome with spike-in normalization on B...
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DESeq in libraries with spike-in samples
Hi all,
in our experiment we have control triplicates as well as two different knockouts. for each of the conditions the total RNA as well...
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ChIP-Seq Challenge
Community ChIP-Seq Challenge 1.0
Hello Folks,
We need your help! Yes you!
Here is an experiment in open...