Dear all,
We are pleased to announce our upcoming online course “Generalised Linear Models in R”, taking place 25–29 May.
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Generalised Linear Models in R course
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Question about using limma for differential analysis
For the Differential analysis I'm using "limma" R package. I'm using RSEM log2 transformed counts data from XENA browser. A matrix "U"...
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Statistical analysis gene expression correlation
Dear all,
I've got a zinc finger protein (znf479) that binds on 920 genes in the brain. We hypothesize that the binding of this protein...
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Finding significantly different ChIP-seq peak length?
Hello,
I have a simple data analysis question.
Say I have a protein ChIP-seq data comparing three replicates of wild-type...
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Advanced interaction contrast in DESeq2
Hi everyone,
I am currently working on some RNA-seq data in DESeq2 and I would like to know if I can transform a specific...
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Analysis of RNA-seq data without replicate.
Hi guys!
Is there any reliable method for statistical analysis of RNA-seq data with no replicates?? If there are only one test and control...
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DEG analysis between distant species
Hi all,
I am recently thinking about how to implement DEG analyses on two distant species. My idea is to get the expression values (like...
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How to visualize microbiome datasets ?
Hi,
I have 16S rRNA datasets produced by Mothur and I want to plot my data and get publictaion quality figures (stacked bars, heatmaps,...
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Advice for Statistics in Gene Expression??
Hi Everyone,
I am a beginner in RNA-seq and am working on a project dealing with differential gene expression. I am trying to compare the...
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Hi from Montpellier (France)
Dear SEQanswers community,
I a postdoc working on Rice and its pathogen Magnaporthe.
I have just started RNAseq project...
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RNASeq Differential Gene Expression with two-sample Mann-Whitney test on FPKM?
I am interested in performing differential gene expression analysis of RNASeq data in two conditions with a number of biological replicates for each condition....
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Significance of Overlapping Regions
I have two bed files of genomic coordinates. (say 12000 entries in one, 1000 in the other) I know how many unique bases in the genome are covered by each...