Hello,
I have DNA reads mapped in the genome reference in the format .SAM, i need to calculate dN/dS in each ORF.
How can I calculate...
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Calculating dN/dS with NGS data
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Program that gives summary of substitutions
Hey,
I am looking for a program that gives a summary of base substitutions from a vcf file, e.g. number of A --> T, C --> T, etc.... -
Roche Jr Sequencing Kit Polymerase
While prepping enriched beads for a GS Jr run last week, I messed up the addition of the polymerase. That's what I get for trying to hurry. Anyway, to... -
454 Pyrosequencing error rate assesment tool
Hi,
Is anyone aware of some software or documented algorithm for estimating (separately) the error rate of PCR and sequencing for 454 data,...
