Roche Jr Sequencing Kit Polymerase

[lisa.brown]

While prepping enriched beads for a GS Jr run last week, I messed up the addition of the polymerase. That's what I get for trying to hurry. Anyway, to fix my mistake I just opened a second sequencing kit and used the polymerase from it. Never prep a run without back-up reagents in the freezer, right?! Well, now I have a full sequencing kit without any polymerase... Grrrr...

Any suggestion on which polymerase, at what concentration, to use as a substitute for the one that comes in the kit? I called Roche Tech Support and DNA polI at normal concentration was suggested as a substitute, but they couldn't tell me exactly what is in their kit. Just hoping someone has experience with this and could tell me exactly what will work.

[pmiguel]

Just ask Roche to replace the polymerase.
You may be their only Roche Jr customer left, so what else will they do with all that polymerase?

[lisa.brown]

Yeah, I tried that already. I would need to order, and pay for, a whole new kit. They do need to move as many as possible now... Too bad the price hasn't dropped.