17 results in 0.0029 seconds.
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BWA SW's equivalent for MEM's "-C" option?
Please delete that thread, I should use the latest version of bwa, there is the "-C" flag for BWASW as well... -
Agilent SureSelect XT and Illumina flowcell saturation
Does anyone know if the SureSelect XT kits have enough probes in a single reaction to saturate a single lane in an Illumina HiSeq flowcell (200-400 million... -
Wierd base calling on MiniSeq run
We recently ran some libraries on the MiniSeq (2 x 150 bp Mid Output kit) and got some weird traces for the %base graph (see image attached). The other... -
Agilent SureSelectXT - input
Hello,
I have been using the recommended input of 3ug for Aglient SureSelectXT but when I QC post covaris/post clean-up, I am left with...Last edited by sward; 10-21-2016, 03:24 AM. -
SureSelect Wash 2
Hi everyone! I had a failure with sureselect exome enrichment - no DNA in captured sample. I reread manual, then i read Faircloth (2013) "Target...Last edited by salyamoff; 10-13-2016, 09:15 PM. -
Key Considerations for Whole Exome Sequencing
http://blog.genohub.com/key-consider...me-sequencing/
- Should I choose Whole Genome Sequencing or Whole Exome Sequencing
- How
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Illumina Rapid capture vs. Agilent SureSelect Exon
I am currently using Illumina's Nextera Rapid capture Enrichment kit for WES Library prep but am about to start a new project and was wondering whether... -
Sureselect disappearing DNA
Hello All
We are doing the Sureselect protocol (XT all exon V4) with 3ug of DNA, after size selection we have around 1ug left for the end... -
Sureselect kits
Hello,
Does anyone know the difference between the SureSelect Primer in the Sureselect library prep kit ILM and the InPE primer 1.0 in the Sureselect... -
PCR program for Sureselect libraries
Hello,
After hybridisation in the Sureselect protocol, there is a PCR amplification. What program do you use for this step?
The... -
Agilent automated library preps-Low yields after pre-hybridisation PCR
Hiya,
I'm having problem with low yields following the pre-hybridisation PCR. I'm starting with the recomended 3ug and the fragments are the correct... -
GATK UnifiedGenotyper SNP calling with Agilent 50Mb target enrichment
I am trying (and failing) to call SNPs using a reference file (.BED) specifically for the Agilent 50Mb Sureselect enrichment sequencing. I am using the... -
Analyzing Targeted Resequencing data with Galaxy
Hi!
I am having problems with my sequencing results, but I am a newbie at this; so I am thinking there is something wrong with my analysis. So... -
SureSelect target enrichment. Optimal hybridization time
Hello,
We just started using Agilent's sureselect target enrichment system in the lab. Their protocol says hybridization time is 24 hours,... -
Sequencing/Capture problems
Has anyone experienced that the sequences generated after the capture are mostly those not included in the capture??
I have just looked at 1/10...
