Hi all
I recently made ATAC-seq libraries on two new cancer cell lines and I don't see nucleosomal pattern as I used to see in my previous library....
13 results in 0.0029 seconds.
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ATAC-seq library fragment size troubleshooting
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How does the "Illumina DNA PCR-Free Prep (Tagmentation)" work?
Has somebody here figured out how the new "Illumina DNA PCR-Free Prep (Tagmentation)" library preps work?
https://www.illumina.com/p... -
demultiplex BCL data with two different barcoding systems
Hi,
I was wondering if anyone has experience in demultiplexing BCL files from Illumina with two different barcoding systems in one go?... -
Nextera DNA Flex Library Prep - TWB
Does anyone know the composition of the Tagment Wash Buffer in the Nextera DNA Flex Library Prep (20018704) protocol? Does it contain alcohol? PEG? ... -
Nextera DNA Exome Tagmentation Peaks
I'm new to the library prep side of sequencing and I've been having trouble getting my post-tagmentation, post-amplification libraries to match the profile... -
hiChIP – library prep – library size distribution ok?
Dear all,
I have performed a library prep of hiChIP samples according to the Mumbach paper (Mumbach et al. 2017). As the tagmentation and Tn5 amount... -
A few questions about ATAC-seq library preparation. Thank you!
Hi everyone,
I've done my first ATAC-seq library preparation recently and had a few questions about the result. I would be very grateful... -
Nextera XT tagmentation problem
Hello all,
I need some help with Nextera XT library prep from bacterial genome.
DNA were extracted using QIAmp kit with QIAcube HT robot... -
Tagmentation DNA Buffer (TD)
Hey folks,
This seems a slim chance, but here goes: Does anyone have a recipe for the DNA Tagmentation Buffer that comes with Nextera DNA... -
adaptors and primers Nextera kit: modified or not?
Hi,
I am using Nextera XT kit from Illumina but tried to order the adaptors from another vendor, since the sequence is disclosed. Unfortunately,... -
Storage of tagmented samples
Hi,
Does anyone know if the quality of results is affected by storage of a sample after tagmentation and clean-up at -20 or how long a sample can...Last edited by emilia_r; 10-22-2013, 11:41 AM. -
Nextera vs Nextera XT: what´s the difference?
Hi all,
I tried to figure out the difference between Nextera and Nextera XT kit (for low input). With the Nextera kit I was never able to get good... -
tagmentation of the 3'-end of cDNAs: which sequences are added?
Hi!
I want to sequence the 3'-end of all transcripts of a cell using the Nextera kit.
The tagmentation rxn is done on full-lenght ds cDNA...
