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Inexpensive, high throughput DNA methylation quantification
I have a probe list (varies between 200 to 7,000 probes) from the Illumina 450K Beadchip that I'd like to quantify as cheaply as possible. Needs to be... -
Multiplexing PCR for targeted sequencing
Dear All,
Has anyone done multiplex PCR for targeted sequencing on MiSeq?
What software have you used to design the primers?... -
Best approach for targeted PCR resequencing on MiSeq from RNA
Hi everyone,
I need to amplify selected regions from my RNA samples and resequence with MiSeq.
I am unclear to what is the... -
Nimblegen MHC SeqCap EZ
Is anyone doing HLA sequencing with the Nimblgene SeqCap EZ Human MHC design? Or are you doing HLA with your own custom pull-down kit? If so, I would... -
De novo assembly of transcripts originating from specific target regions
Hello everyone,
After alignment of my paired-end RNAseq data, I have isolated some regions of interest and extracted reads mapping to these...
