Hi everyone,
I'm currently working on a mRNA-seq which was performed by a private company. After obtaining the data, I'm filtering and trying to...
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About using or not FPKM threshold after DESeq2
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Basemean threshold
I have an rna seq dataset and I am using Deseq2 to find differentially expressed genes between the two groups. However, I also want to remove genes in...
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What is your threshold for QC?
Hello everybody,
I am really new to this field and trying to learn to use Galaxy.
Just wondering if there is any criteria...
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RPKM threshold estimation
Dear All,
I have a doubt in the calculation of False postitive rate while checking for FPKM threshold in a RNAseq experiment.
...
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RPKM threshold
I've just started working with RNA seq data and what I found is a wide range of RPKM values. The problem is that I don“t know which is the best way to...
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Significance of an FPKM value
Curious what some other people might be thinking about what I am seeing in the literature these days. Am I wrong that the general contention that people...
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RNA-Seq cutoff formula..
Hi,
I posted similar question under RNA sequencing, but after second thought Bioinformatics section might be better suited.
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threshold
In Mortazavi et al (Nature Methods,2008. 5: 621) they used 5 RPMK as threshold and reported genes that had RPMK more than 5.0.
Is this applicable...