Hi,
I posted similar question under RNA sequencing, but after second thought Bioinformatics section might be better suited.
After calculating RPMK values for transcripts, how do I calculate the cutoff values (under which the transcript is considered not expressed)? Is this calculated manually with a formula? OR is it calculated by RNA-Seq software?
I used rSeq and it just provides RPMK values, there's no statistics. So, I'd greatly appreciate if you can guide me..
Thanks..
I posted similar question under RNA sequencing, but after second thought Bioinformatics section might be better suited.
After calculating RPMK values for transcripts, how do I calculate the cutoff values (under which the transcript is considered not expressed)? Is this calculated manually with a formula? OR is it calculated by RNA-Seq software?
I used rSeq and it just provides RPMK values, there's no statistics. So, I'd greatly appreciate if you can guide me..
Thanks..