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high percentage of reads mapping to tRNA?
How do you interpret this? What does it mean for wet-lab side of things, does it inform any step that went right or wrong? This is for a total RNAseq...
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Useful to run total RNA along with RIPseq?
I have some RNA isolated from an RNA immunoprecipitation. I was wondering if I should also sequence the (input) total RNA and what advantage/use this...
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Article on totalRNA isolation kits
http://www.biomedcentral.com/1472-6750/14/94/abstract
BackgroundThis study compared the performance of five commercially available kits...
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Need a RNA 6000 Pico Total RNA Kit?
Hi Guys,
I feel weird posting this here but we have a complete RNA 6000 Pico Total RNA Kit (http://www.genomics.agilent.com/en/Bioanalyz...
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Wacky BioAnalyzer Results
Hi All
Has anybody ever seen anything like these?
I'm asking this question in as many forums as possible since nobody around me...
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RNA-Seq biology
As far as I can see the literature is using total RNA for RNA-Seq analysis. This is fine if you're doing gene expression level. But there are numerous...