Hello, every one!
I have got the transcript downloaded form NCBI. Also we have got the RNA-Seq data from illumina platform. Now we want quantify...
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Quantification for transcirpt without reference genome.
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ChIP-seq and Peak calling
I have a simple question that I would like some opinions on. If a transcription factor is highly expressed (protein) in one cell and say now that the...
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RMA Nimblegen Data
Hello SeqAnswers community,
I'm rather new to analyzing microarray data, so please forgive my ignorance. I have a couple of questions....
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which is the main transcript
For a specific gene, how can I know which is the main transcript in the ensembl? there is a list of few transcripts and not always the first one or the...
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Combining DNASeq and RNASeq data
I have multiple RNASeq datasets from one plant species which does not have an assembled reference genome. But I have access to three whole genome re-sequencing(DNASeq)...
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Coverage of a transcript and accuracy of DE
Hi all,
quite a basic question, but I find they can be the hardest to find answers for.
If I have transcripts with greater...
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Metatranscriptome analysis pipeline
Hello there!
I've just started analysing bacterial metatranscriptome Illumina data. I'm a bit confused on the pipeline to follow. That's what I...
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Underdispersed mapped RNA-Seq reads
Hello all,
I am working on analyzing RNA-Seq samples for differential expression and am perplexed by the distribution of my data, which...
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Assembly using phrap
Hej!
I am working on a data my group received from MWG. They were using, as far as I know 454 sequencing to sequence short 3' fragments...