Hello,
So, from Ensembl FTP, we can download a transcriptome file which is a FASTA file containing the header info, which is transcript name,...
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Getting transcriptome sequence from RNA-seq reads in FASTA
Last edited by barbarian; 03-03-2016, 08:32 PM. -
Navigating De-Novo RNA data
Hey guys, I just finished a de-novo transcriptome assembly with Trinity and Trinotate. These programs produce individual fastq files that will have the... -
Tophat2 giving unreasonably long junctions
I'm using Tophat2 to align my RNA-seq reads. Many of the junctions produced by Tophat have extremely long introns and span multiple genes in my dataset.... -
Problems using GATK for variant calling
Hi,
I am busy working with Eucalyptus transcriptome data generated using the Illumina platform. I mapped the paired end reads using TopHat...
