Hi All,
I have recently run an ATAC-seq library on PBMCs that came back with 82% reads mapping to E.coli and only 5% mapping to the human...
5 results in 0.0021 seconds.
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Contamination of E.coli reads in ATAC-seq library
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Wierd base calling on MiniSeq run
We recently ran some libraries on the MiniSeq (2 x 150 bp Mid Output kit) and got some weird traces for the %base graph (see image attached). The other... -
Tn5
Does anyone know of another source of Tn5 transposase other than
FC 121 1030 form Illumina.
Cheers -
Trying to remove nextera transposase sequence using cutadapt and fastqc
Hello everybody,
We just launched a nextseq500 run recently.
I analyzed it with Fastqc and I obtained an error at the adapter content...Last edited by ClemBuntu; 01-21-2015, 08:04 AM. -
Nextera Problem: Odd fragment size after gel selection
Hi guys, I'm constructing a PE library by using the Nextera DNA library Prep Kit (Illumina compatible). However, when I cut down the 300-350bp, 450-500bp,...
