Hello all,
I have created an illumina truseq library on bacteria by first performing ribozero rRNA depletion kit and then performing a truseq...
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strong 140bp peak following RNA library construction
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TruSeq mRNA Elution Buffer
Has anyone tried homemade Elution Buffer (ELB) with a TruSeq stranded mRNA kit? We've run out of buffer before other kit contents, and as the kit is expired...
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mRNA library - final loading concentration for MiSeq?
Probably this has been covered before, but with the information scattered in numerous threads I'll ask again... What final loading concentrations are...