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bwa mem stringent mapping
I'm trying to map quality trimmed Illumina 100bp reads to a reference in order to get the leftover reads which are potentially not the reference (bacteria/fungi)....
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Are these reads aligned to my reference (BWA)?
I am currently extracting unaligned reads with the syntax below:-
According to http://picard....Code:samtools view -f 4 *.bam
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Extract fastq files of unaligned reads with Bowtie 2
Hi everyone
I am using Bowtie 2 (2.0.0-beta2) to do alignments on the output reads of an Illumina HiSeq 50bp paired-end RNA-seq experiment....
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BLAT - uniquely mapped reads/multiple hits
Hi all,
I wanted to know if there was a simple way to filter the output.psl from BLAT to obtain a file containing only the uniquely mapped...