Dear community,
I wonder whether anybody ever did a benchmarking of in silico PCR tools? I couldn't find any benchmarking so far.
Are there...
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in silico PCR benchmarking
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Non-ascii characters in bam file causes htseq-count error
Hello everybody,
I have aligned bam files (using STAR version 2.6.1a_08-27) and want to quantify them using htseq-count (version 0.9.1)....
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Script to calculate Dipeptide composition
Hi all, I would like to use a script (python, shell, or perl) to calculate the percentages of dipeptide composition from multiple protein sequences (fasta...
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UMI-tools running error- help needed :)
Hi everyone,
I am trying to work with UMI-tools. After installation, I tried to follow the example they provide.
After executing...
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dineshkumarsrk started a topic How to reverse complement the nuclotides, if the coordinates are in reverse order?in GeneralHow to reverse complement the nuclotides, if the coordinates are in reverse order?
I have the series of coordinates in id.txt file, whose coordinates sequences are in genome.fasta file. The coordinates of id.txt file is shown below,...
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Automated script for paired end sequencing
Hello everybody,
I have a folder with paired end sequencing data (divided by the asterisk to make group clearly visible here). I would like...
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MAKER prerequisites and dependencies
Hello all,
I'm a biologist trying to be a bioinformatician, and I'm doing a local installation of MAKER on my own, which requires a bunch...
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Cuffmerge error file never signals "done"...is this normal?
Hello all,
I'm fairly new to the bioinformatics world and am trying to combine several gtf files with cuffmerge. My shell script is as...
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How to blastx on Drosophila melanogaster (taxid:7227) with raw data?
Here is some background. I am doing a preliminary study on some raw (cleaned) data. I want to find positive hits (I already used blastn to find negative...Last edited by hlyates; 05-08-2015, 06:54 AM.
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nested for loop to concatenate fastq files
My pooled PE RNA-Seq data was demultiplexed by the sequencing facility, so the data I receive is a directory with sub directories for each sample that...
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find files which contain a string
Hi members,
Pardon me if this looks a trivial doubt.
I know this has been asked several times on stackoverflow, unix and many other...Last edited by bio_informatics; 03-04-2015, 05:27 AM.
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System Administrator for Bioinformatics
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Max Delbrück Center, Berlin Institute for Medical Systems Biology, Bioinformatics platform is seeking an experienced individual...Last edited by altuna; 06-27-2014, 07:24 AM.
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Unix System Administrator for the UC Davis Bioinformatics Core (Davis, CA)
The UC Davis Bioinformatics Core at the UC Davis Genome Center provides data analysis, infrastructure services, and training to the research community....
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Parsing file to retrieve specific pattern
I have a txt file with several rows. Each row contains several words separated by space. I want to print the values which have pattern like amenXXXXXXX...