Hi Folks
I am curious as to how many people have set up and run MGI sequencers. I have an SP-100 and a G-50 sequencer. What was easy, what was difficult?...
15 results in 0.0021 seconds.
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Setting up and using MGI platforms
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Normalizing RNA-seq data vs Normalizing qRT-PCR
I am working on a project to validate the results of RNA-seq differential expression analysis using qRT-PCR. I use BioRad software (CFX Maestro). I'm... -
vcftools and bcftools disagree
Hello,
I'm trying to impute my data using HRC (sanger server). For that I validated and annotated my vcf using GATK and then prepared... -
Where can I find sequencing data with known genotype?
Hello all,
I'm trying to find sets of public sequencing data with verified/known genotype in order to test my bioinformatics pipeline (for... -
RNAseq independent validation by qPCR
Hi All,
I was wondering if anyone is aware of any RNAseq papers which validated using qPCR (or similar) on a new set of samples? i.e. samples A,B,C,D... -
Validation of Splicing Results from cufflinks-cuffdiff pipeline
Hi
I am shortlisting my candidates for validation using qPCR/RTPCR of my results from two different splicing pipelines(DEXSeq & Cufflinks/Cuffdiff).... -
MEME centrimo peak validation with known sequence
Hi!
I have done a chip-seq experiment and obtained a number of peaks. Using MACS v1.4 I obtained 1262 peaks with IDR < 0.01 between... -
Necessity of strand-specific RT-qPCR to validate RNA-seq hits?
Hi all,
I'm very new to all of this NGS stuff and I am concerned that my ignorance on a particular issue is going to be debilitating...again.... -
Complete genome validation
Hi guys,
I have a question regarding how to validate a completed bacterial genome. The sequencing technology used was the Illumina GAIIX,... -
Validation by genotyping arrays (SNP arrays) - why only heterozygous SNPs?
Hi,
I am trying to validate the variants I found using whole genome sequencing . The standard practice, I have seen in the two publications...Last edited by ak352; 09-16-2013, 04:53 AM. -
Validation algorithm.
Hi.
I work in the development of algorithm for work with differential expression with data from mRNA-Seq.
Now I need validate this... -
RNA-Seq / Sanger validation data set
Hello
So I want to evaluate some variant calling workflows and one thing that would be nice to have is a validated (Sanger) / published... -
Validation of Illumina on other NGS platforms
Hi we are currently doing our Illumina variant validations using sanger sequencing. Has anyone used another platform like 454 or PGM to validate their... -
wet lab Validation of a large number of SNPs
Hi
AFAIK
you can validate SNPs obtained by Hi-seq by
microarray,
Sanger Seq,
NGS seq on a different platform ... -
Generating input for Amosvalidate
Hi,
I'm using ABySS to assemble 14 FASTQ PE files into a 300MB assembly. I wanted to use AmosValidate (http://bit.ly/pSVBr8) for validation...
