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Clive Brown's Technology Talk from London Calling 2018
For the last 4 years I've attended Oxford Nanopore Technologies' yearly London Calling conference. One of the interesting bits of this conference is the... -
Nextera XT low yield
Hi!
I am using the XT Nextera kit with environmental samples. DNA was extracted and purified by phenol/chlo and column (to remove EDTA).
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Moderately Low Yield ChIP
Hello all,
I have been doing qChIP successfully for a while now, and I am trying to make the venture into ChIP-seq. My problem is that... -
Agilent automated library preps-Low yields after pre-hybridisation PCR
Hiya,
I'm having problem with low yields following the pre-hybridisation PCR. I'm starting with the recomended 3ug and the fragments are the correct... -
low yield of SOLiD templated beads
Hi
We have been facing problems with solid 3 plus to come up with sufficient beads for deposition. We hope to sort this out before moving to solid... -
Where does all the DNA go?
The standard Roche protocol for shotgun library construction asks for 10 ug of input DNA to yield a few million templated beads for sequencing. Rule of...
