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  • RNA-PET and paired-end RNA-Seq

    Sorry, I am a green hand.

    Could any person kindly help me point out the difference between them. Are they the same technology? If not, which one is better to detect the fusion events in transcriptome?

    By the way, have u heard the concept of Transcriptome-induced Cimeras(CITs)? what's ur opinion?

    Thanks.
    Failure is an option, but fear is not!

  • #2
    RNA-PET is a method to sequence the 5' and 3' end of a transcripts.
    Paired-end RNA-seq is a method to sequence of a segment of the transcripts from both end of the segment.

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    • #3
      Originally posted by HZAU_xf View Post
      RNA-PET is a method to sequence the 5' and 3' end of a transcripts.
      Paired-end RNA-seq is a method to sequence of a segment of the transcripts from both end of the segment.
      I think I have got it. This strategy finely fits the Illumina's GA to detect the structure varients in the transtription level. Moreover, there are paired-end seq and mate paire seq methods based on the length of the insert sequence between the two ends, 100~200bp and 1k~1.5K bp. The company says that it would be better to combine short with long which can be certain to identify all different sizes and types of varients.

      Reference:www.illumina.com/Documents/.../datasheet_genomic_sequence.pdf

      Thanks for your help anyway!
      Failure is an option, but fear is not!

      Comment


      • #4
        Are you working on the NGS data. If you want to learn more about RNA-PET, you can take a few minutes to read this article http://www.ncbi.nlm.nih.gov/pubmed?t...d%203%20%C2%A2

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