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Greetings! I am looking for an RNA-Seq data set to play with. Especially 454 transcriptome data. Any ideas? By the way, anybody did a study to run the same RNA-Seq with both 454 and Illumina? Thanks! -Simon
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A good place to start is the Omnibus Gene expression database at http://www.ncbi.nlm.nih.gov/geo/ there are plenty of uploaded sequencing files, you can also try the SRA at NCBI
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Like all molecular biology applications, next-generation sequencing (NGS) workflows require diligent quality control (QC) measures to ensure accurate and reproducible results. Proper QC begins at nucleic acid extraction and continues all the way through to data analysis. This article outlines the key QC steps in an NGS workflow, along with the commonly used tools and techniques.
Nucleic Acid Quality Control
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