![Confused](https://www.seqanswers.com/core/images/smilies/confused.png)
hello ,everyone
I am trying to run bowtie with the solid sequence data flower9_1_F3.csfasta and flower9_1_F3_QV.qual
I built the bowtie-build -C index successfully and moved the generated index files to the Bowtie indexes subdirectory. I run the command:
bowtie -n 3 --best --strata -a -e 150 -p 4 ../cDNA_reference/TAIR9_cdna -C -f flower9_1_F3.csfasta -Q flower9_1_F3_QV.qual --un fllower9-unmap > flower9-map &
and the output i got is an error showing on console
[wanglei@mu01 data]$ [wanglei@mu01 data]$ Too few quality values for read: 425_2031_2008_F3
are you sure this is a FASTQ-int file?
terminate called after throwing an instance of 'int'
Too few quality values for read: 425_2042_1847_F3
are you sure this is a FASTQ-int file?
terminate called after throwing an instance of 'int'
Too few quality values for read: 1277_2042_2013_F3
are you sure this is a FASTQ-int file?
Could you please help me to sort out this issue?
thanks
wanglei
Comment