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  • arrchi
    Member
    • Mar 2011
    • 46

    #1

    cufflinks without reference stopped at chrM

    My code is as simple as this:
    Code:
    cufflinks -o test accepted_hits.bam
    The bam file was generated by tophat. Both tophat and Cufflink are the latest version (1.3 and 1.03).

    Without a reference, cufflinks stop and here is the last output:

    Processing Locus chr9:141152985-141153035 [***************** ]> Processing Locus chrM:0-16571 [************************ ] 97%
    I tried cufflinks (v 1.0.2). I saw the same problem.

    However, if I use cufflinks with a reference, the program runs smoothly without this problem.

    Any suggestion?

    Thanks in advance.
    Last edited by arrchi; 06-09-2011, 10:14 AM.
  • arrchi
    Member
    • Mar 2011
    • 46

    #2
    I checked cufflinks output , there is no chrX, chrY and chrM in the three output files. But my input bam file does have these three chromosome info.

    Any ideas? please!
    Last edited by arrchi; 06-09-2011, 10:15 AM.

    Comment

    • arrchi
      Member
      • Mar 2011
      • 46

      #3
      One of my several cufflinks programs does finished after stuck at chrM for about one day. So i guess it just needs a little bit more time and patience.

      Comment

      • steven
        Senior Member
        • Aug 2009
        • 269

        #4
        OK, that's probably because chrM is often overloaded with reads

        Comment

        • baritone
          Junior Member
          • Jun 2015
          • 5

          #5
          The problem is still there

          And I'm posting this in the year of 2016

          Comment

          • GenoMax
            Senior Member
            • Feb 2008
            • 7142

            #6
            Originally posted by baritone View Post
            And I'm posting this in the year of 2016
            Even with latest cufflinks version?

            Comment

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