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  • MerFer
    Member
    • Sep 2009
    • 21

    #1

    tophat fusion

    Hello Tophat user,

    I run the tophat fusion on my RNA seq data. I want to load my accepted_hits.sam to the IGV browser. So trying to convert the sam file to the bam file with samtools view. But I get some errors because of the fusion reads.

    [samopen] SAM header is present: 25 sequences.
    [sam_read1] reference 'chr10-chr2' is recognized as '*'.
    Parse error at line 37: invalid CIGAR operation
    Aborted

    Is there any other way to convert the file, without removing the fusion reads?

    Thanks.

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