Dear all,
I've currently got a set of small RNA samples on the SOLiD 4 and am wanting to perform some small RNA analysis.
My samples are canine and therefore I have obtained the cfa.gff file from miRBAse as recommended in the SOLiD RNA2MAP manual.
My question is how do I ensure I have the correct genomic reference fasta file (also required)? From NCBI I note there are chromosome.fa and chromsome_ref.fa - what is the difference in these please?
Best wishes,
D
I've currently got a set of small RNA samples on the SOLiD 4 and am wanting to perform some small RNA analysis.
My samples are canine and therefore I have obtained the cfa.gff file from miRBAse as recommended in the SOLiD RNA2MAP manual.
My question is how do I ensure I have the correct genomic reference fasta file (also required)? From NCBI I note there are chromosome.fa and chromsome_ref.fa - what is the difference in these please?
Best wishes,
D