what is the difference between exome and transcriptome ? how does their assembly differ ?
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The transcriptome consists of all that is transcribed, ie all RNA species including mRNA, miRNA, tRNA, lncRNA, etc. It is usually studied through samples of RNA. You can get the basic idea by reading this: http://en.wikipedia.org/wiki/Transcriptome
The exome refers to the protein coding portion of the genome. It is usually studied through samples of DNA. I'd recommend reading this article for an example of exome sequencing and its applications: http://www.ncbi.nlm.nih.gov/pmc/arti...1/?tool=pubmed
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by seqadmin
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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04-22-2024, 07:01 AM -
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by seqadmin
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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04-04-2024, 04:25 PM -
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