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  • #31
    Nice, lots of answers. We have two computer for analyzes in our lab, but they are suitable to run gene expression analysis (Tophat, cufflinks etc), not de novo assembly, like the one I have to do. Before we buy them I was using Amazon cloud. We also have a cluster that I can use in our university, the only problem is that I have to schedule the time I will be using and with limitation. I wanna run a couple different methods (trinity, velvet, abyss) and play with the parameters to see what give me the best assembly.
    What about the Amazon high memory instances, it is not expense actually and the biggest one is:
    High-Memory Quadruple Extra Large Instance 68.4 GB of memory, 26 EC2 Compute Units (8 virtual cores with 3.25 EC2 Compute Units each), 1690 GB of local instance storage, 64-bit platform
    The advantage of using it is that I can access whenever I want, but I might end up using our local cluster.
    Thanks

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