My project is to find SNP from Sanger sequences. I've never done before. Here is some steps I could think of to achieve my purpose. Please suggest appropriate free tools/software for each step. Please let me know if I miss any steps.
Step 1: quality trim a ABI file (to Fasta).
Step 2: align with a reference genome (input Fasta, output Sam?)
Step 3: parse the output file to retrieve SNP?
Step 1: quality trim a ABI file (to Fasta).
Step 2: align with a reference genome (input Fasta, output Sam?)
Step 3: parse the output file to retrieve SNP?
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