Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • yaximik
    Senior Member
    • Apr 2011
    • 199

    Still unresolved question?

    Hi everyone,

    I was browsing previous topics in hope to find a way to generate GoldenPath-type annotation file (AGP format?) to feed into GS Reference Mapper, similar to refGene.txt that can be dowloaded from UCSC Genome Browser site (for human genome). A few questions like conversion of a GenBank annotatoion file still remain unanswered, but these are one year old inquiries.

    Basically, I am looking for a way to generate GoldenPath-type refGene.txt or snp130.txt for bacterial genomes using GenBank entires. I found a tool to generate .GFF file from GenBank, which can be fed in Genome Browser to make custom tracks, but it is not obvious that something on the site can be used for conversion to the GoldenPath format. GS Reference Mapper seems to take only GoldenPath annotation files, but not GFF. Are any further developments on the conversion problem?

Latest Articles

Collapse

  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
    07-01-2026, 11:43 AM
  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 07-02-2026, 11:08 AM
0 responses
21 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-30-2026, 05:37 AM
0 responses
22 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-26-2026, 11:10 AM
0 responses
21 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-17-2026, 06:09 AM
0 responses
54 views
0 reactions
Last Post SEQadmin2  
Working...