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  • biofreak
    Member
    • Jun 2011
    • 44

    CanFam2 data RNAseq samples and GenomicRanges

    hi All,
    I am processing RNAseq samples for canFam2. I used the following commands from GenomicRanges package that gives me only 1218 genes. (because by default refGene for canFam2 retains only known genes.)

    txdb <- makeTranscriptDbFromUCSC(genome = "canFam2", tablename = "refGene")
    TranscriptDb object:
    | Db type: TranscriptDb
    | Data source: UCSC
    | Genome: canFam2
    | UCSC Table: refGene
    | Type of Gene ID: Entrez Gene ID
    | Full dataset: yes
    | transcript_nrow: 1218
    | exon_nrow: 11256
    | cds_nrow: 10922
    | Db created by: GenomicFeatures package from Bioconductor
    | Creation time: 2011-08-08 12:56:52 -0400 (Mon, 08 Aug 2011)
    | GenomicFeatures version at creation time: 1.4.2
    | RSQLite version at creation time: 0.9-4
    | DBSCHEMAVERSION: 1.0

    To get all the genes, I changed the table name to "xenoRefGene" but that does not fetch the "gene Ids". Also, the number of transcripts (229403) looks abnormally large.

    TranscriptDb object:
    | Db type: TranscriptDb
    | Data source: UCSC
    | Genome: canFam2
    | UCSC Table: xenoRefGene
    | Type of Gene ID: no gene ids
    | Full dataset: yes
    | transcript_nrow: 229403
    | exon_nrow: 1110585
    | cds_nrow: 747878
    | Db created by: GenomicFeatures package from Bioconductor
    | Creation time: 2011-08-08 12:51:29 -0400 (Mon, 08 Aug 2011)
    | GenomicFeatures version at creation time: 1.4.2
    | RSQLite version at creation time: 0.9-4
    | DBSCHEMAVERSION: 1.0


    please help.

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