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  • #1

    Questions about Scriputre RPKM

    Hi.

    I only have two RNA-Seq samples. Could I directly use RPKM ratio to find out differentially expressed genes between these two samples? RPKM values were generated by Scripture.

    Wish your help! Thanks a bunch!
    Tags: None
  • #2
    Yes, RPKM was designed to normalize gene depth, read number.. but the ratio is somehow arbitary, without significant level.

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