miRDeep2

[asaleh]

Hello,

I Am currently using miRDeep to to analyze small RNA-seq data from the Solid 4.

However I am having trouble aligning expression between samples.

Does anyone have experience with miRDeep that could suggest a method or suggest another mapping/analysis program.

Thanks,

Anthony

[NicoBxl]

what do you mean by aligning expression ? the reads ?

[asaleh]

sorry I was not more clear.

I mean aligning the reads for each microRNA from different samples. The output does not lend itself to that as far as I can figure out. Just wondering if others had encountered this problem and figured out how to solve it.

[Kamy24]

Hi,

I am trying to identify miRNA from fastq file. I am using miRDeep2 for this purpose. As mentioned in the in the tutorial regarding the mature and precursor sequences of miRNA, I have downloaded it from miRBase version 21. While running the mirdeep2.pl command im getting this error in the report.log file.

" #Starting miRDeep2 /home/titan4/mirdeep2_0_0_8/src/miRDeep2.pl reads_collapsed.fa hg38.fa reads_collapsed_vs_genome.arf mature_new.fa none precursor_new.fa -t H.sapiens

miRDeep2 started at 12:3:54

mkdir mirdeep_runs/run_24_06_2016_t_12_03_54
testing input files

started: 12:3:54 sanity_check_mature_ref.pl mature_new.fa

[1;31mError: [0mproblem with mature_new.fa Error in line 5.177: The sequence



contains characters others than [acgtunACGTUN]

Please check your file for the following issues:

I. Sequences are allowed only to comprise characters [ACGTNacgtn]. II. Identifiers are not allowed to have withespaces.

Can some one help.

[natallah]

It looks like you likely have whitespaces in the headers of your fasta file, which mirDeep does not allow. See https://www.biostars.org/p/135035/ for various options on how to remove these

[gurjeet]

Novel MicroRNA prediction with Mirdeep 2.

I recently done small RNA sequencing of my endocrine tumour samples. I got result analysis with Mirdeep2 . I am not master in bioinformatics so , i outsourced the analysis. The company have predicted some novel micrornas. They have used mirdeep2 software for predicting novel microRNA.

I am facing problem in selecting novel microRNAs from small RNA sequencing. Is it necessary to have high mirdeep2 score with high mature read count. Those sequence with high mirdeep2 score ( like 3168) & mature read count (456) have non significant RANDFOLD p value. I am confused in this part.

I read in mirdeep2 documentation in which the score cut was from -10 to 10.but i am getting mirdeep2 score of like 200 , 361. I am really very confused. Then i started comparing total read length , loop read length. But still confused.

Please guide me regarding mirdeep2 software and please help me its parameters for selecting novel microRNA.I will be very thankful to you.