Hi everyone,
I have just started annotating a transcriptome of a non-model organism (reads derived from a 454 sequencing) but would like to know what is the best algorithm to do it. I am wondering about blasting the assembled sequences using blastn to identify rRNA genes and genomic DNA and then blast the contigs left using blastx. Does it make sense to you?
Many thanks.
I have just started annotating a transcriptome of a non-model organism (reads derived from a 454 sequencing) but would like to know what is the best algorithm to do it. I am wondering about blasting the assembled sequences using blastn to identify rRNA genes and genomic DNA and then blast the contigs left using blastx. Does it make sense to you?
Many thanks.
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