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hi folks i have to do the transcriptome analysis using illumina sequencing so could you please tell me ho to convert the sra file into fastQ formate
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You should think about moving your question somewhere else, as the topic is by no means related to your question... -
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The mentioned program is part of the SRA Toolkit and can be found here:
Help on how to use the fastq-dump can be found here:
Good luck!Comment
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Hi there. I have been using the SRA toolkit and have had no trouble using the fastq-dump tool but I have also been trying to get the SRA files into SFF format to use sff_extract for use with MIRA3 assembler. Unfortunately I can't get it to work and as a complete novice (both with bioinformatics and LINUX) working more or less alone on this I would really appreciate any help if anyone has used it. Any alternate approaches would also be appreciated for assebling and analysis of 454 EST data.
Thanks, seenstevoComment
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