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  • SRA to FASTQ

    hi folks i have to do the transcriptome analysis using illumina sequencing so could you please tell me ho to convert the sra file into fastQ formate

  • #2
    You should think about moving your question somewhere else, as the topic is by no means related to your question...

    Comment


    • #3
      Originally posted by ulz_peter View Post
      You should think about moving your question somewhere else, as the topic is by no means related to your question...
      Moved, good catch.

      Comment


      • #4
        fastq-dump

        Originally posted by ramashankar16 View Post
        hi folks i have to do the transcriptome analysis using illumina sequencing so could you please tell me ho to convert the sra file into fastQ formate

        Comment


        • #5
          The mentioned program is part of the SRA Toolkit and can be found here:


          Help on how to use the fastq-dump can be found here:


          Good luck!

          Comment


          • #6
            Hi there. I have been using the SRA toolkit and have had no trouble using the fastq-dump tool but I have also been trying to get the SRA files into SFF format to use sff_extract for use with MIRA3 assembler. Unfortunately I can't get it to work and as a complete novice (both with bioinformatics and LINUX) working more or less alone on this I would really appreciate any help if anyone has used it. Any alternate approaches would also be appreciated for assebling and analysis of 454 EST data.
            Thanks, seenstevo

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