Tweet
coverage is around 10X with unique and filtered reads.
-
-
Hi there,
I am confused with my RNASeq analysis result. After mapping the reads to Human reference using TopHat, 62% is mapped to the intronic or intergeneric region.
Here is short summary about my experiment:
1. Extract total RNA using Qiagen RNAmini kit
2. Prepare library using Illumina TrueSeq protocol
I am wondering maybe this is due to the high amount of premature mRNA. How do you think? Does anyone have the simillar experience? Any comment is welcome.
Thank you so much for your attention,
LahomanComment
-
What portion of the reads mapped to intergenic regions? Were those regions close to or distant from genes? Perhaps the DNA digestion step in your RNA extraction failed and you actually sequenced a mix of DNA and mRNA. Check the genomic regions where the reads map to for poly-A runs.Comment
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment