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  • dacotahm
    Member
    • Oct 2011
    • 28

    Velvet-Oases Memory Problem

    I'm using a small data set to test Velvet-Oases of about a million (av. length 200bp) reads. Pretty soon we're going to get a much larger data set, but the memory requirements for the much smaller assembly is crazy.

    When I turn on read-tracking to run Oases with 15G of memory it will max out and kill at about 60-70% nodes visited. How would this ever work with 10-100 million reads if the largest-memory instance I can create has about 70G of memory?

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  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM
  • SEQadmin2
    From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
    by SEQadmin2


    Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


    The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
    ...
    06-02-2026, 10:05 AM

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