unique reads
oh ok. so im using the s_#_sequence.txt file in MAQ, i presume this is just as good as the export.txt file? im using the 'match' command with default settings and 'assemble' with -q 10. Is this sufficient to get alignments consisting only of unique reads, i.e reads that map to only one position?.
thanks for your help
-
You might be better off using filtered reads from the export file since MAQ gives non unique reads a low mapping quality and thus do not contribute to SNP calling. Also even though the documentation states that sorted.txt contains only uniquely aligning reads I have seen cases where a sorted.txt file contains reads which DO map to more than one location.Leave a comment:
-
s_#_sorted.txt files
hello,
im a NGS newbie and i have some solexa data from their pipeline v1.3. can the s_#_sorted.txt files be used in MAQ and how?. i believe these files contain reads that map to the genome uniquely.
thank you
-
This year’s Advances in Genome Biology and Technology (AGBT) General Meeting commemorated the 25th anniversary of the event at its original venue on Marco Island, Florida. While this year’s event didn’t include high-profile musical performances, the industry announcements and cutting-edge research still drew the attention of leading scientists.
The Headliner
The biggest announcement was Roche stepping back into the sequencing platform market. In the years since...03-03-2025, 01:39 PM -
The human gut contains trillions of microorganisms that impact digestion, immune functions, and overall health1. Despite major breakthroughs, we’re only beginning to understand the full extent of the microbiome’s influence on health and disease. Advances in next-generation sequencing and spatial biology have opened new windows into this complex environment, yet many questions remain. This article highlights two recent studies exploring how diet influences microbial...02-24-2025, 06:31 AM
Leave a comment: