Hi.
We did RNA paired-end sequencing. After alignment analysis, I found there were such reads. One end were with multiple alignments, while the other end with unique alignment. How to treat these reads? Should I eliminate them before inputting the alignments into Cufflinks or Scriputure?
Wish your suggestions! Thanks very much in advance?
We did RNA paired-end sequencing. After alignment analysis, I found there were such reads. One end were with multiple alignments, while the other end with unique alignment. How to treat these reads? Should I eliminate them before inputting the alignments into Cufflinks or Scriputure?
Wish your suggestions! Thanks very much in advance?
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