Sample short read data set?

[ECO]

Anyone have a sample data set that they'd be willing to share? I'll host!

[apfejes]

A sample of what type of data?

[ECO]

Ideally for my application, short read genomic data (ie, solexa/abi). I'm playing around with some of the software packages listed in this forum and need some data!

[apfejes]

Sorry, I should have been clearer in my message: what experiment type? ChIP-Seq, Genome shotgun, Transcriptome shotgun, etc. Getting generic data is easy - getting results for a particular type of data may not be.

[ECO]

Genome shotgun, or better yet amplicon enriched genomic reads. ie. not transcriptome data nor ChIP.

Actually now that I think about it it would be nice to have sample data sets for all applications, but I only have 6TB of bandwith per month!

[apfejes]

Hrm.. I'm not sure we have many (good quality) genome shotgun data sets kicking around that I'd be able to get permission to release. At least, I personally don't have any, yet. I'll poke around, though, and maybe I can find something for you. If you don't mind poor quality, just for playing around, that might be feasible.

[ECO]

That would be great. I'm not concerned with base quality or recalling, etc. I'm working on analysis platforms...and it's no fun to just randomly generate short reads.

Let me know!

[apfejes]

Hi ECO,

I brought this up yesterday, and was told that there is no point in making any data available. Supposedly there are open repositories (NCBI?) collecting and making this data available. I spent the last 15 minutes looking for said repositories, but couldn't find anything remotely like I expected.

On the other hand, doing a Google search for ".seq.txt", which is the common file name of sequences produced using the Illumina pipeline, I came up with a set of Histone ChIP experiments that the BC Genome Science Centre has made available anyhow:

http://www.bcgsc.ca/downloads/histone

I did confirm that they were intentionally released, so I'm sure there's no problem with using them. On the other hand, I don't know a lot about these particular sets of data. I do know they're not new: they were analysed a while ago, and I've seen several presentations on this information over the last year or so.

The files themselves are post-base calling, but not yet aligned. They may be good for testing aligners or whole pipelines. (Then again, they're old, they may not be a good test for the latest Illumina software - Interested parties can try that themselves.)

I suspect the wig files (where available) were created with Findpeaks 2.1.x, though I haven't verified this.

Cheers,

Anthony

[sci_guy]

Data DVD

Applied Biosystems have a sample data DVD of S. suis reads together with a few compiled executables (for UNIX), some Perl code and a workflow document.
Data DVD

[ECO]

Anthony & sci_guy, thanks much! I'll take a look!

I'll probably be starting a thread soon about the best OSS solutions for putting together one's own analysis platform. Not really to support an instrument, but to analyze a small number of runs for a specific project.