an error of reading fastq by tophat - SEQanswers

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aquleaf

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Join Date: Mar 2010

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an error of reading fastq by tophat

10-23-2011, 07:25 AM

Hi.

I have two fastq files of paired-end sequencing which contain 32,442,740 each. But tophat reported as follows:
Left reads: min. length=49, count=32,422,792
Right reads: min. length=49, count=32,362,999
Does anyone have any idea about what happened?

Wish your help! Thanks very much!

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