Hi Folks
I'm working with fastq files from a few sequencing platforms - Illumina, SOLiD, Ion Torrent, 454. I'm running these files through MIRA and Velvet for de novo assemblies. I have been running these read sets through FastQC and can see where the read qualities drop off in the various data sets. Is there a tool that will allow me to trim the 3' ends of the reads before submitting them to a de novo assembler? I was hoping pre trimming might yield better contigs.
Thanks!
I'm working with fastq files from a few sequencing platforms - Illumina, SOLiD, Ion Torrent, 454. I'm running these files through MIRA and Velvet for de novo assemblies. I have been running these read sets through FastQC and can see where the read qualities drop off in the various data sets. Is there a tool that will allow me to trim the 3' ends of the reads before submitting them to a de novo assembler? I was hoping pre trimming might yield better contigs.
Thanks!