Hi
I have downloaded standalone blast and formatted a nucleotide database. I wish to use tblastn to compare Arabidopsis proteins against it. I made a db folder in the folder where blast is saved and changed to that directory then ran the following command:
tblastn -query tair1prot -db petunia -evalue 0.01
However I receive an error:
BLAST Database error: No alias or index file found for the nucleotide database [petunia] in search path [C:\Program Files\NCBI\blast-2.2.25+\db;C:\Program Files\NCBI\blast-2.2.25+\db;]
As you can see I think the program is taking the file path of the current directory and then adding the directory where databases are stored on to the end. This would mean the db directory and the index file are not found. If I change directory to C:\ for instance then the errror would be C:\;C:\Program Files\NCBI\blast-2.2.25+\db;
How can I stop this from happening so the database can be located correctly? What do you think I might be doing wrong?
Thanks
Jon
I have downloaded standalone blast and formatted a nucleotide database. I wish to use tblastn to compare Arabidopsis proteins against it. I made a db folder in the folder where blast is saved and changed to that directory then ran the following command:
tblastn -query tair1prot -db petunia -evalue 0.01
However I receive an error:
BLAST Database error: No alias or index file found for the nucleotide database [petunia] in search path [C:\Program Files\NCBI\blast-2.2.25+\db;C:\Program Files\NCBI\blast-2.2.25+\db;]
As you can see I think the program is taking the file path of the current directory and then adding the directory where databases are stored on to the end. This would mean the db directory and the index file are not found. If I change directory to C:\ for instance then the errror would be C:\;C:\Program Files\NCBI\blast-2.2.25+\db;
How can I stop this from happening so the database can be located correctly? What do you think I might be doing wrong?
Thanks
Jon
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