The number of LowQual using ApplyRecalibration as the same as the LowQual in raw.vcf.
LowQual number does not change!!! and a little PASS change to TruthSensitivityTranche99.00to99.90.
should I filtering itself based on the VQSLOD values? I followed the pipeline:
LowQual number does not change!!! and a little PASS change to TruthSensitivityTranche99.00to99.90.
should I filtering itself based on the VQSLOD values? I followed the pipeline: