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ERROR: [bcf_sync] incorrect number of fields..

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SEQanswers June Challenge Has Begun!

The competition has begun! We're giving away a $50 Amazon gift card to the member who answers the most questions on our site during the month. We want to encourage our community members to share their knowledge and help each other out by answering questions related to sequencing technologies, genomics, and bioinformatics. The competition is open to all members of the site, and the winner will be announced at the beginning of July. Best of luck!

For a list of the official rules, visit (https://www.seqanswers.com/forum/sit...wledge-and-win)
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  • #16
    Thanks Sacrolfur, I'll give that a try.

    Comment


    • #17
      Try to use both commands separately and write output here.

      Comment


      • #18
        using -u when running samtools mpileup

        Comment


        • #19
          I got the same problem. I tried the verson 1.0, 0.1.19, 0.1.18 and none worked.
          Any one can help to solve this problem.

          Here are my command and error message:

          samtools mpileup -ugf F.fna F.sorted.bam > F.bcf
          [mpileup] 1 samples in 1 input files
          <mpileup> Set max per-file depth to 8000
          [bcf_sync] incorrect number of fields (6 != 5) at 67:234
          Segmentation fault

          Comment


          • #20
            I figure out it problem by update the bcftools from 0.1.17 to 1.1.

            Comment


            • #21
              hello,all,I think I have solve this problem:
              both nohup and ">" would add stdout and stderr to output file,which cause that problem。

              so,
              "samtools mpileup -ugf F.fna F.sorted.bam -o F.bcf"
              instead of
              "samtools mpileup -ugf F.fna F.sorted.bam > F.bcf"

              Comment


              • #22
                Same here, the bcftools that is compiled within samtools is old, and when I compiled a fresh version from github, this error vanished.
                Sam Hokin
                Computational Scientist, Carnegie and NCGR

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