Hello,
I used FIMO to find transcription factor binding sites for all the TFs in Jaspar database (130TFs). Only 78 out of 130known JASPAR motifs had predicted motifs by FIMO.
I was wondering how this can be possible. For around 50 known curated motifs not to have binding sites in the genome. Does P-val has something to do? Even then its highly unlikely that there is not even a single significant binding site for those 50TFs.
Any suggestions or comments??
I used FIMO to find transcription factor binding sites for all the TFs in Jaspar database (130TFs). Only 78 out of 130known JASPAR motifs had predicted motifs by FIMO.
I was wondering how this can be possible. For around 50 known curated motifs not to have binding sites in the genome. Does P-val has something to do? Even then its highly unlikely that there is not even a single significant binding site for those 50TFs.
Any suggestions or comments??