Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Hena
    Member
    • Nov 2009
    • 19

    #1

    dindel, exomes and chromosome 10

    Hi all,

    I run dindel as part of workflow which I do for dna seq, which large percent is exome samples. I've noticed something quite odd with regards to dindel stage 3.

    I run stage 3 parallel using cluster. I split the input with '--numWindowsPerFile 1500' currently. Most of the executions take between 20 and 50 minutes to run. Some take more. However I've noticed that in many cases there is one that takes a lot more time (can take several days). That seems to happen in situations where the chromosomal position is in chr 10 and 42Mb. Has anyone else noticed anything similar? There seems to be high number of haplotypes printed in log*, but I did check this for each file in one sample and mean haplotype of all lines per file doesn't seem to follow execution time that file.

    * eg the it has something like this:
    [empiricalDistributionMethod] Number of haplotypes: 136

Latest Articles

Collapse

  • SEQadmin2
    16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by SEQadmin2




    NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
    Yesterday, 06:46 AM
  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 10-28-2026, 09:57 AM
0 responses
25 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-28-2026, 09:51 AM
0 responses
16 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 11:13 AM
0 responses
71 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 10:48 AM
0 responses
48 views
0 reactions
Last Post SEQadmin2  
Working...