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  • shawpa
    Member
    • Aug 2011
    • 73

    samtool sort: truncated file error?

    I was trying to sort a bam file using samtools. The original bam file is about 34.4GB. After I ran the sorting, the output file was only 12.9GB. If it worked correctly I don't see where over half the data went. I checked a log and it says

    [bam_sort_core] truncated file. Continue anyway.
    [bam_sort_core] merging from 103 files...

    I'm not sure what this means. I think it might be a memory issue and its creating tmp dir where there isn't enough space maybe. I wasn't able to find any posts with this error already.

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
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    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
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    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

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