Hi
I am trying to test BWA with 454 read data larger than 200 nt using the bwasw option and the hg19 as indexing reference.
BWA generates a ooutpu.sai which i try to convert to sam format and here is the problem.
bwa gives the4 following message
[bns_restore_core] fail to open file 'hg19.nt.ann'. Abort!
Aborted
The point is that I have not idea about what bwa ask me for the file hg19.nt.ann or what is the hg19.nt.ann file. This file is not generated with the other index files when I run the index function, so i am confusing.
I checked the forum about other similar messages and surprinsingly I have found very little (almost nothing clarifying to my doubt) about this.
Can anyone clarify me if this file xxx.nt.ann is normal output of bwa and how I can create it for converting a sai file to bam
Thank you in advance.
I am trying to test BWA with 454 read data larger than 200 nt using the bwasw option and the hg19 as indexing reference.
BWA generates a ooutpu.sai which i try to convert to sam format and here is the problem.
bwa gives the4 following message
[bns_restore_core] fail to open file 'hg19.nt.ann'. Abort!
Aborted
The point is that I have not idea about what bwa ask me for the file hg19.nt.ann or what is the hg19.nt.ann file. This file is not generated with the other index files when I run the index function, so i am confusing.
I checked the forum about other similar messages and surprinsingly I have found very little (almost nothing clarifying to my doubt) about this.
Can anyone clarify me if this file xxx.nt.ann is normal output of bwa and how I can create it for converting a sai file to bam
Thank you in advance.
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