Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Mapping reads with adapter sequences using MAQ

    I am trying to test pair end mapping with 3' adpter sequence and please let me know how the alogorithm handles the situation.

    The adapter sequence is stored in a text file. The adpter sequence is about 7 bps length. If the paired reads are mapped against the reference genome with option -d which contains 3' adapter, how does the algorithm handle the mapping situation.

    Will it just eliminate 7bps found in the reads as contaminants and eliminate the adpter portion of the reaad from mapping?

    What if both read1 and read2 does not contain the adapter sequence?

    If I am not concerned about the -a option (ie) Maximum outer distance for a correct read pair which has the default value as 250 is not of my concern and how to disable the -a option.

    Anyone can explain on how to interpret the outputdata?

    Thanks.

  • #2
    Hi Can anyone suggest a tool to map pair end reads with adpter sequence? Thanks.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Recent Advances in Sequencing Analysis Tools
      by seqadmin


      The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
      Yesterday, 07:48 AM
    • seqadmin
      Essential Discoveries and Tools in Epitranscriptomics
      by seqadmin




      The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
      04-22-2024, 07:01 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Yesterday, 07:17 AM
    0 responses
    12 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 05-02-2024, 08:06 AM
    0 responses
    19 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-30-2024, 12:17 PM
    0 responses
    20 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-29-2024, 10:49 AM
    0 responses
    29 views
    0 likes
    Last Post seqadmin  
    Working...
    X