Hi all,
I have some Illumina paired-end (100 bp) read data and have seen this very useful page: http://intron.ccam.uchc.edu/groups/t...Sequences.html
It has some primer adapter sequences and primer sequences in it. My question is, do I have to remove any additional sequences than the ones on this page and the primers I used to amplify my cDNA, such as the index primers? Is the sequence for index primers the same as the PE sequencing or PCR primers given in the link above, with just the index tag added?
Should I worry about reverse complementing all of these and removing those sequences as well?
Liz
I have some Illumina paired-end (100 bp) read data and have seen this very useful page: http://intron.ccam.uchc.edu/groups/t...Sequences.html
It has some primer adapter sequences and primer sequences in it. My question is, do I have to remove any additional sequences than the ones on this page and the primers I used to amplify my cDNA, such as the index primers? Is the sequence for index primers the same as the PE sequencing or PCR primers given in the link above, with just the index tag added?
Should I worry about reverse complementing all of these and removing those sequences as well?
Liz
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