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  • Dbuch
    Junior Member
    • Apr 2012
    • 3

    #1

    Why are there different numbers of gene_id and exon_id in a txdb database from UCSC?

    I'm trying to run DEXseq but am having trouble with the newExonCountSet function because my gene_id list and exon_id list are different sizes. When I unlist the mm10 gene_id dataset from UCSC annotated with Ensembl Genes (ensGene) it yields 351,862 gene_ids even though the corresponding exon_id dataset contains only 350,630 IDs. I had a similar problem when trying to annotate the UCSC database with RefSeq Genes (tablename = 'refGene'). Any thoughts on why there is a difference and how I can get the Gene_ID and Exon_ID datasets to match up? Thanks!

    Code:
    library("GenomicRanges")
    library("Rsamtools")
    library("GenomicFeatures")
    library("rtracklayer")
    
    txdb <- makeTranscriptDbFromUCSC(genome="mm10", tablename = "ensGene")
    
    #Getting rid of strand information
    exonRangesList <- exons(txdb)
    strand(exonRangesList) <- '*'
    exonRangesNoStrand <- split(exonRangesList)
    
    ExonIDs <- exons(txdb, vals=NULL, columns=c('gene_id', 'exon_id'))
    GeneIDList <- elementMetadata(ExonIDs)
    GeneIDVector <- GeneIDList$gene_id
    ExonIDVector <- GeneIDList$exon_id
    GeneIDVector2 <-unlist(GeneIDVector)
  • Dbuch
    Junior Member
    • Apr 2012
    • 3

    #2
    Update: When I make the txdb using the code below (getting the data from Ensembl instead of UCSC) you get equal numbers of gene_id and exon_id.

    Code:
     txdb <- makeTranscriptDbFromBiomart(biomart="ensembl", dataset="mmusculus_gene_ensembl")

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